The pyromark q24 mdx is designed as a standalone instrument, which makes it easy to place anywhere in the lab. Pyromark q24 mdx accessories and reagents qiagen online shop. The product is intended solely for use on the pyromark q24 mdx system. Reagents for pyrosequencing pyromark go to the product. Suitable for detecting atb resistance, microbial identification, snps, etc. Pyromark q24 advanced and pyromark q24 mdx are based on the pyromark q24. Performance of different analytical software packages in. Highresolution quantification of di, tri, or tetraallelic mutations genotyping and quantification of indels. The instructions and safety information in the user manual must be followed to ensure. Qiagen kit handbooks and user manuals are available at. Pyromark q24 software user guide 072009 7 pyromark q24 software the pyromark q24 system is a complete solution comprising instrument, vacuum workstation, chemistry, and software. Strict compliance with the instrument user manual and this handbook is required for. Light signals emitted are detected by 24 ccd sensors one sensor per well thereby eliminating signal crossover. Assays were created according to manufacturers instruction.
Pyromark q24 user manual 012009 11 1 safety information before using the pyromark q24 system, it is essential that you read this user manual carefully and pay particular attention to the safety information. Pyromark q24 mdx user manual 012016 11 1 safety information before using the pyromark q24 mdx, it is essential that you read this user manual carefully and pay particular attention to the safety information. New progress in pyrosequencing for automated quantitative. The product is intended to be used by professional users, such as technicians and physicians who are trained in in vitro diagnostics procedures, molecular biological techniques. These parameters can be manually changed by the operator, but they can not be lowered below 5 rlu. Sample to insight pyrosequencing workflow template preparation new progress in pyrosequencing for genotyping applications 34 annealing of sequencing primer sequencing primer only binds to biotinylated pcr strand ssdna is needed for binding binding done o manually pyromark q96 id, q24, q24 mdx, q24 adv o automated pyromark q48. Print bookmark share pdf 3996kb english format file size language download get adobe reader other available languages turkce dansk francais deutsch.
Pyrosequencing was performed on a pyromark q24 mdx instrument according to the manufacturers user manual qiagen, hilden, germany. The pyromark q24 mdx dispenses reagents and nucleotides to each well with precision. The pyromark q24 mdx uses proven pyrosequencing technology for realtime. Pyromark gold q96 cdt reagents 6 x 96 pyromark q96 cpg line1 192 pyromark q48 advanced reagents 4 x 48 pyromark q48 advanced cpg reagents 4 x 48 pyromark q48 magnetic beads 300 pyromark q48 autoprep starter kit pyromark q48 discs 50 pyromark q48 absorber strips 100 pyromark pcr kit 200 pyromark pcr kit 800 qx e. Pyromark q24 is a fully integrated system that provides realtime sequence information, and is highly suitable for epigenetic research and genetic analysis. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the. Show details download files 15 sort options sort alphabetically pyromark q24 mdx method 0014 en instrument methods file for use with pyromark q24 mdx software, pyromark gold q24 reagents, and pyromark q24 cartridges marked with method code 0014. For the quantitative analysis of genetic or epigenetic.